A STUDY ON THE EFFECT OF TIME ON DEGUMMING OF SILK AND PRODUCTION OF ISLAND LIKE MORPHOLOGY IN REGENERATED SILK FIBROIN.
Silk is considered as a biomaterial with basically two types of protein embedded and overlapped, namely, fibroin and sericin. Fibroin is the fibrous protein and sericin is the gummy sheath which holds the fibroin fibres together in a core sheath structure in a ratio of 1:3. The present study focuses on the degumming study of the silk cocoons where the removal of sericin is an important factor whether it is textile or Pharmaceutical research. The cocoons were subjected to boiling temperature in 0.02 M sodium carbonate for different time frames for 30 mins, 45 mins, and 60 mins and checked the % removal of sericin with the help of degumming loss % equation. Then the best sample with maximum removal of sericin is forwarded to prepare regenerated silk fibroin where an issue of Island like morphology is procured which is considered as a significant finding in case of drug delivery concepts. The characterization part was done to give a testimony to the fact obtained through research work. To see the effect of sericin removal (Degumming) from fibroin in silk cocoons, Scanning Electron Microscopy (SEM) was performed and for Regenerated silk fibroin, along with SEM, Atomic force microscopy (AFM) was also performed.